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Sperm retrieval techniques

When sperm is not present in the ejaculate a Testicular Biopsy or a Percutaneous Epididymal Sperm Aspiration (PESA) may be recommended in order to collect sperm for treatment.


Sperm collected through these procedures can then be used in assisted insemination (IUI) and in vitro fertilisation (IVF). If possible, we will obtain enough sperm for freezing as well. This may mean that you will not need another sperm retrieval procedure if future IVF cycles are undertaken.


Testicular Biopsy

The procedure of testicular biopsy is performed with a fine needle. This involves placing a fine needle into the testes and withdrawing some of the tubules from within the testes which contain live sperm. The procedure is usually performed under local anaesthesia.

Testicular Biopsy

Percutaneous Epididymal Sperm Aspiration (PESA)

The procedure of Percutaneous Epididymal Sperm Aspiration (PESA) is performed by passing a fine needle into the epididymis and aspirating sperm.

Testicle Biopsy - Percutaneous Epididymal Sperm Aspiration (PESA)


The man may feel some discomfort in the 24 hours following these procedures, but simple pain killers, like Panadeine, are usually sufficient to overcome this. Men can usually go to work the day after this procedure.